Figure 4.

Puf3p-mediated decay of CYT2 mRNA is conditionally regulated. Shown are representative northern blot analyses of the decay of the CYT2 transcript from a WT and a puf3Δ strain grown in media supplemented with dextrose (Dex), ethanol (EtOH), galactose (Gal) or raffinose (Raff). Minutes following transcriptional repression are indicated above each set of blots, with the half-lives (T_1/2) and standard error of the mean (SEM) determined from multiple experiments shown graphically to the right of each set of blots.