Figure 6.
Puf3p-mediated decay of COX17 mRNA is conditionally regulated. Shown are representative northern blot analyses of the decay of COX17 (A–C) and STE3 (D) transcripts from a WT strain and a puf3Δ strain grown in media supplemented with dextrose (Dex), ethanol (EtOH), galactose (Gal) or raffinose (Raff). Minutes following transcriptional repression are indicated above each set of blots, with the half-lives (T1/2) and standard error of the mean (SEM) determined from multiple experiments shown graphically to the right of each set of blots. The average half-life of STE3 mRNA is represented graphically with SEM determined from multiple experiments.