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. 2013 Dec 25;42(6):3833–3845. doi: 10.1093/nar/gkt1333

Figure 3.

Figure 3.

Interactions of MUS81 with EME2 and EME2B. (A). Extracts were prepared from T-REx-293 cells expressing SFEME2 or SFEME2B from a tetracycline-inducible promoter and probed by western blotting for the indicated proteins. The SFEME1 or SFEME2 proteins were immunoprecipitated using FLAG antibodies and the immunoprecipitates were probed for the presence of MUS81. (B). Western blots showing the presence of EME2 in the indicated cell lines, with and without siRNA treatment. Beta-actin was used as a loading control. (C). The U2OS cells expressing SFEME2 from a tetracycline-inducible promoter were treated with cisplatin. The SFEME2 and MUS81 proteins were detected by immunofluorescence using anti-FLAG and anti-MUS81 antibodies, respectively. DNA was visualized by DAPI staining.