Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 26;42(6):3857–3870. doi: 10.1093/nar/gkt1331

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — Pseudouridine formation by TruB variants under multiple-turnover conditions. Tritium-labeled tRNA^Phe (0.95 μM) was incubated with TruB (0.2 μM) at 37°C, and pseudouridine formation was quantified at selected time points with the help of a tritium release assay. (A) Two-hour time courses with TruB wild type (closed circles), TruB D90E (closed squares), TruB D90N (closed triangles) and TruB D90A (closed diamonds). (B) Thirty-hour time courses with TruB wild type (closed circles) and TruB R181K (open squares) to confirm detectable activity (no enzyme control, open circles).