Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Apparent rates of tRNA binding to TruB wild type and variants
| TruB | kapp2, s−1 |
|---|---|
| Wild type | 4.2 ± 0.5 |
| D90E | 1.5 ± 0.7 |
| D90N | 1.9 ± 0.4 |
| D90A | 2.0 ± 0.4 |
| R181K | 1.9 ± 0.5 |
| R181M | 3 ± 1 |
| R181A | 4 ± 1 |
Apparent rates (kapp2) for the strong fluorescent increase were determined by 3-exponential fitting of stopped-flow time courses for TruB wild type and 2-exponential fitting for the TruB variants (Figure 5). kapp2 represents the average apparent rate from at least five time courses ± standard deviation.
