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. 2014 Jan 20;42(6):e48. doi: 10.1093/nar/gkt1402

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Engineered GAL1 promoter variants. (A) Measuring the effects of Zif268 binding site location and spacing on Z₃EV-mediated gene induction. Schematic cartoons (not drawn to scale) illustrate the tested promoter architectures and bar plots are measurements of GFP intensity in the presence or absence of inducer following ∼12 h of induction. (B) Measuring the effects of 5′-UTR sequence on Z₃EV-mediated gene induction, by replacing the GAL1 5′-UTR with that of SOL1 or GAL80. Error bars represent the standard deviation of fluorescence from three independent colonies/cultures.