Figure 6.

(A) A previously known ssu72-restricted transcript (SRT) in the promoter of OPY1 is detected by SMORE-seq in WT cells under normal growth conditions (45) (top two panels). A novel antisense ncRNA that may share a bidirectional promoter with LPT1 is shown below. (B and C) Widespread occurrence of bncRNAs (antisense ncRNAs at bidirectional promoters). Genes were clustered by K-means clustering (K = 5, repeat = 1000) of bncRNA signal in a range 300 to 50 bp upstream of TSS. Genes in the indicated tandem arrangement are shown in (B), and (C), in the divergent arrangement. Divergent genes whose TSS are closer than 300 bp are excluded in (C). The vertical line represents the TSS of downstream genes. The number of tandem genes and divergent genes in this heat map are 2401 and 1635, respectively.