Figure 3.

Using SMRT Sequencing to analyze nuclease activity. (A) SMRT sequencing produced very similar results to standard TOPO sequencing over a range of mutation rates from ∼20% to ∼76%. Error bars are 90% confidence intervals. S2/S5 NK and S2/S5 NN are the TALENs targeting beta-globin compared in this study. S116/S120 and J7/J8 are NK-TALENs targeting beta-globin and CDH1, respectively (30). (B) Comparison of the range and frequency of different sizes of indels observed in cells treated with TALENs or ZFNs. The observed frequencies of the different sizes are normalized to the frequency of the most common indel size for each nuclease type.