Table 2.
pH dependent thermal melting results for rHP1–PNA triplexes and rHP1 alone
| pH | rHP1–P8 |
rHP1–J3 |
rHP1–L3 |
rHP1 | |||
|---|---|---|---|---|---|---|---|
| Tm1 | Tm2 | Tm1 | Tm2 | Tm1 | Tm2 | Tm2 | |
| 5.5 | (84.6) | 84.6 | NC | 83.1 | (82.9) | 82.9 | 82.4 |
| 6.0 | (84.5) | 84.5 | NC | 84.1 | (84.0) | 84.0 | 83.1 |
| 6.5 | 62.6 | 83.8 | 36.1 | 84.6 | (83.8) | 83.8 | 84.2 |
| 7.0 | 51.9 | 84.6 | 32.6 | 84.6 | (84.0) | 84.0 | 83.5 |
| 7.5 | 38.1 | 83.3 | 29.9 | 84.8 | 64.1 | 83.9 | 84.3 |
| 8.0 | 26.9 | 84.6 | 29.2 | 83.8 | 55.7 | 83.9 | 83.6 |
| 8.5 | <20.0 | 83.9 | NC | 84.1 | 52.7 | 84.6 | 83.6 |
| 9.0 | <20.0 | 82.7 | 29.2 | 82.6 | 47.6 | 83.3 | 82.5 |
Buffers contain 200 mM NaCl, 0.5 mM EDTA, 20 mM MES (pH 5.5 and 6.0) or HEPES (pH 6.5–9.0). All melting temperatures are shown in °C. Values shown in parentheses are for melting traces with Tm1 merging with Tm2. NC, the melting is not clear due to a broad transition.