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. 2013 Dec 25;42(6):4080–4093. doi: 10.1093/nar/gkt1345

Figure 6.

Figure 6.

Inter-molecular PRE for the PmrAC–box1 DNA complex. (A) A portion of 2D 1H, 15N TROSY-HSQC acquiring in the paramagnetic state (in red; EDTA is chelated with Mn2+) overlaid with that in the diamagnetic state (in black; EDTA is chelated with Ca2+) for PmrAC–box1 complex with dT-EDTA at THY4. (B) and (C) The ratios of peak intensity from the paramagnetic to diamagnetic state with dT-EDTA at THY4 and THY28, respectively. The proline and the severely overlapped residues are shown with gray bars. In (B), the residues with intensity ratio <0.5 are in red. In (C), the residues with intensity ratio <0.75 are in orange. (D) The best structural model from HADDOCK colored according to the PRE results. For spin-labeling at THY4, the Mn2+ and residues with intensity ratio <0.5 are in red. For spin-labeling at THY28, the Mn2+ and the residues with intensity ratio <0.75 are in orange. The EDTA is shown with green sticks.