Skip to main content
. 2014 Jan 9;42(6):4100–4112. doi: 10.1093/nar/gkt1360

Figure 3.

Figure 3.

The interaction between POLRMT and TFAM is dependent on the NTE. (A) Isothermal titration calorimetry experiments of TFAM-binding to POLRMT (B) TFAM-binding to POLRMTΔNTE or (C) TFAMΔCTE binding to POLRMT performed at 25°C. In all three cases, the top panel represents the heat signal for injections of TFAM or TFAMΔCTE into the calorimeter cell containing POLRMT or POLRMTΔNTE. The bottom panel displays the integrated heat for each injection after peak integration and subtraction of basal values fitted to a simple single-site binding model (solid line). The stoichiometry (N) and binding constant (KD) for the interaction between TFAM and POLRMT are shown in the bottom panel. No thermal effect was observed for the putative interaction between TFAM and POLRMTΔNTE, or between TFAMΔCTE and POLRMT.