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. 2014 Jan 20;42(6):4043–4055. doi: 10.1093/nar/gkt1392

Figure 2.

Figure 2.

Scp160 binds to mRNAs. (A) Western blot of the immunoprecipitation of myc9-tagged Scp160p, Scp160p truncated for KH domains 13 and 14, She2p and Khd1p using anti-myc antibody. T—total extracts, FT—flowthrough, IP—immunoprecipitate. (B) Scp160p binds CCW14, AGA1, PRY3, POM34 and MSB2 mRNAs. Immunoprecipitates were subjected to qRT-PCR and analyzed for bound ASH1 RNA (positive control in She2p IP), CFT1 RNA (negative control) and candidate RNAs CCW14, AGA1, PRY3, MSB2 and POM34. Specific enrichment was calculated as ratio of the signal in the target IP to the mock IP (untagged wild-type strain). Statistical significance (Student’s t-test) compared with the KH domain truncation is indicated: *, P < 0.05; **, P < 0.01. Data are presented as mean ± standard deviation, n = 3.