Figure 6.

SRp38 protects DT40 cells from ER stress-induced apoptosis. (A) WT, KO and KO-re cells were treated with the ER stress-inducer tunicamycin (TM, 2 ug/ml) for 28 h. Apoptosis was measured by propidium iodide staining and flow cytometry (right panel). (B) Cells were treated with TM (2 ug/ml) for 16 h and allowed to recover in medium for 1–3 days. The number of surviving cells was determined by trypan blue exclusion. The error bars represent the SD. (C) WT and KO cells were subjected to TM-induced ER stress for the indicated times. Immunoblotting was performed with whole cell extracts using antibodies against SRSF10, phospho-eIF2A and β-actin.