Figure 2.

(a) Titration curves demonstrating the equilibrium number of binders n_P along a chain of binding sites versus the change in energy on binding for the monomer (a) and dimer (b) situations for a number of different concentrations c. Points represent values determined from simulation, and the lines are the theoretical predictions assuming for (a) and for (b). This demonstrates that the equilibrium behavior of these systems can be described using straightforward statistical mechanics. In (c), the along-the-chain binding correlation function g(j) is plotted versus index distance j. At low concentrations ( nm) there is an abundance of binders at j = 1 due to the dimeric behavior of the binders, along with a correlation hole at j = 2. This oscillation becomes smaller at large c due to the stabilization of singly bound states. .