Figure 6.

(a) Exchange results from the experimental literature [Graham et al. (16)] that demonstrate the concentration-dependent decrease in fluorescence due to a change in the unbinding reaction constant (Used by permission of Oxford University Press). (b) The analogous exchange calculation (n_B versus time t) for a binding energy of , at concentrations relevant to experiments carried out by Graham, et al. (16). Both numerical (N) and analytical calculations (A) are performed, with quantitative matching between the two for in the analytical result. Qualitative matching of both of these to experimental observations in (a) is observed to occur, suggesting that this model captures much of the physics of concentration-dependent binding dynamics. Effective unbinding rate constants (in ) can be extracted from these plots using exponential fits, and the results are in (c). Experimental results come from Graham, et al. (16). Qualitative matching is demonstrated, and better fits may be obtained on adjustment of both and used in the theory.