Table 2.
Bacterial strains and plasmids used in this study
| Strain | Description | Reference or source |
|---|---|---|
|
Pseudomonas syringae |
|
|
| pv. glycinea PG4180 |
Wild type, levan+ |
R. Mitchell |
| pv. phaseolicola 1448A |
Wild type, levan+ |
[33] |
| pv. syringae B728a |
Wild type, levan+ |
[34] |
| pv. tomato DC3000 |
Wild type, levan+ |
D. Cuppels |
|
Pseudomonas syringae pv. glycinea PG4180 | ||
| PG4180.M6 |
Spr, Gmr, lscB lscC mutant of PG4180, levan- |
[10] |
| PG4180.M6(pRA3.1) |
Spr, Gmr, Tcr, lscB lscC mutant of PG4180, containing lscA under control of P
lac
on 3.1-kb PstI fragment in pRK415 |
[10] |
|
Escherichia coli | ||
| DH5α |
supE44 DlacU169 (F80 lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 |
[31] |
| Plasmids | ||
| pRK2013 |
Kmr, helper plasmid |
[35] |
| pLB7.2 |
Apr, contains lscB on 7.2-kb EcoRV insert |
[10] |
| pBBR1MCS |
Cmr, broad-host-range cloning vector |
[36] |
| pBBR1MCS-3 |
Tcr, broad-host-range cloning vector |
[36] |
| pBBR3-500-lscB |
Tcr, lscB gene with −500-bp upstream sequence in pBBR1MCS-3 |
[24] |
| pBBR3(lscA) |
Tcr, lscA gene containing insert from pRA3.1 in PBBR1MCS-3 not under control of P
lac
|
This study |
| pBBR3(lscBUpNA) |
Tcr, fusion of 518-bp upstream region of lscB (including first 48-bp of coding region) and lscA (including start codon and downstream region) in pBBR1MCS-3 |
This study |
| pBBR3(lscBUpA) |
Tcr, fusion construct of 470-bp upstream region of lscB (without N-terminus) and lscA (including start codon and downstream in pBBR1MCS-3 |
This study |
| pBBR3(lscAUpB) | Tcr, fusion of 550-bp upstream region of lscA and lscB (including start codon and downstream region) in pBBR1MCS-3 | This study |
Ap, Ampicillin; Cm, Chloramphenicol; Gm, Gentamycin; Km, Kanamycin; Sp, Spectinomycin; Tc, Tetracycline; r, resistant.