Table 3.
Oligonucleotide primers used in this study
| Oligonucleotides | Nucleotide sequence (5’ - 3’) † |
|---|---|
| pe.BC.PG ~ 150 bp |
GTCACCCATGCGGGCCAGCAG |
| lscB_UpN_f |
CCCAAGCTTCGATTGCAAGCTGATACACGTACC |
| lscB_UpN_r |
TAGGCTAGCTAGAGGACTATTTTTGAG |
| lscA_ORF_f |
CTAGCTAGCATGAGTAACATCAATTAC |
| lscA_ORF_r |
CCCAAGCTTCGGACGTCATCCTGATCGACAC |
| lscB_Up_r |
TAGGCTAGCAATTGATACCTTTAAATAGCTTTGGGAG |
| lscA_Up_f |
CGGGATCCAGCAAAGCGCTGTAAAACAGG |
| lscA_Up_r |
CTACTAGCTAGCGATGATGTCCTTTATTGGCGC |
| lscB_ORF_f |
GCTCTAGATGTCCACTAGCAGCTCTGCTGTAA |
| lscB_ORF_r |
CCCAAGCTTTCAGTATTACGGATACGATGAGC |
| lscA_gly_f |
TAAGCCCGGATTTTCCGGTC |
| lscA_gly_r |
TACTGTATGCGTGCCGCGTT |
| lscA_pha_f |
TCACGCTGACGGCTGACCGC |
| lscA_pha_r |
GCCTACTGTATGCGTGCCGCG |
| lscA_syr_f |
TCACGCTGACAGCTGATCGC |
| lscA_syr_r |
ACCAACGGTATGCGTACCGC |
| lscA_tom_f |
ATCACCCTGACAGCCGACCG |
| lscA_tom_r |
ACCGACAGTATGTGAACCCCGCT |
| lscA_f_RT |
ATGAGTAACATCAATTACGCACCC |
| lscA_r_RT |
TACTTTGGCAATTGCCGCAC |
| lscB_f_RT |
CTCTGCTGTAAGCCAGCTCAA |
| lscB_r_RT |
CGGGTGTGACGCAGGTGTAA |
| gyrA_fw |
CGAAGAGCTGGAAGTGATCC |
| gryA_rv |
GACGCTGAGCCTGATAGACC |
| hexR_fw |
ATGGACCGCGTAAGAAAC |
| hexR_rv | TCAGCCTTGATCCTCGATCGG |
†Restriction sites in the primers are in italics: GAGCTC – SacI, AAGCTT - HindIII, GCTAGC - NheI, GGATCC - BamHI, TCTAGA – XbaI.