Fig. 1.

Effect of intraperitoneal (ip) administration of CCK-8 (0, 0.75, 2.5, 7.5 nmol/kg lean body mass) on 30-min food intake in age- and sex-matched MC4R^−/− and wild-type mice. Data represent means ± SE. Age- and sex-matched mice deprived of food for 18 h received an injection of CCK-8 or vehicle (0.1% BSA) in randomized order immediately before the start of the dark cycle. Food intake was measured 30 min after wild-type (n = 6–8) and MC4R^−/− mice (n = 12–13) received injections of CCK-8 or vehicle and access to food at the onset of the dark cycle. A: response to CCK-8 in wild-type mice. B: response to CCK-8 in MC4R^−/− mice. *P < 0.05 vs. vehicle (VEH). †P < 0.01 vs. VEH.