Fig. 6.
Relative mRNA levels of MC4R, oxytocin, and corticotropin-releasing hormone (CRH) on pPVN neurons that project to the NTS. Data represent means ± SE. A: relative MC4R mRNA levels following analysis of Alexa 488-conjugated CTB-labeled pPVN neurons by quantitative RT-PCR, with the data normalized to GAPDH mRNA levels (44). Relative MC4R mRNA levels in CTB-labeled pPVN neurons were compared with levels in suprachiasmatic nucleus (SCN) as negative control. B: relative oxytocin- and CRH-mRNA following analysis of Alexa 488-conjugated CTB-labeled pPVN neurons by quantitative RT-PCR, with the data normalized to GAPDH mRNA levels. Relative oxytocin- and CRH-mRNA levels were compared with levels in SCN as a negative control. †P < 0.01 vs. SCN.