Skip to main content
. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: J Neurosci Methods. 2011 Feb 1;196(2):303–307. doi: 10.1016/j.jneumeth.2011.01.023

Figure 1.

Figure 1

Immunoprecipitation of DAT and DAT-interacting proteins from rat striatal synaptosomes. DAT was immunoprecipitated from rat striatal synaptosomes. Following SDS-PAGE, the immunoprecipitates were analyzed by Western blotting for DAT (A) or silver-stained for total protein levels (B). A, DAT immunoreactivity was detected in the DAT-immunoprecipitation (IP) lane and was not detected in the immunoprecipitation lane produced with rabbit pre-immune serum (pre-immune). B, numerous discrete protein bands are present in the DAT-IP lane that are not present in the IP lane produced with rabbit pre-immune serum (pre-immune).