Table 2.

Summary of RNA quality control analysis. RNA concentrations, RNA integrity number (RIN), rRNA ratios, positive PCR control (PPC), and reverse transcription control (RTC) used during qRT-PCR of transporter gene expression analysis for mRNA isolated from hypoxic and normoxic rat choroid-retina (CR).

Sample Name	RNA concentrations (ng/μl)	RIN number	rRNA Ratio (28s/18s)	Positive PCR Control (Ct PPC)	ΔCt (Avg Ct RTC - Avg Ct PPC)
Hypoxic-CR1	603	9.0	1.6	18.1 ± 0.17	3.58
Hypoxic-CR2	898	8.9	1.6	20.6 ± 0.12	3.87
Hypoxic-CR3	820	8.6	1.8	19.0 ± 0.29	4.65
Normoxic-CR1	637	9.0	1.7	20.1 ± 0.21	3.86
Normoxic-CR2	523	9.1	1.7	19.1 ± 0.23	3.82
Normoxic-CR3	565	9.3	1.7	18.1 ± 0.19	4.10