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. 2014 Apr;160(Pt 4):682–691. doi: 10.1099/mic.0.074971-0

Table 1. Bacillus subtilis strains used in this study.

Strain Genotype Source/construction†‡
168 trpC2 Laboratory collection
8G5 sinR : : tet trpC2 tyr-1 his ade met rib ura nic sinR : : tet Oscar Kuipers, Groningen, the Netherlands
AM373 sfp+ ermC epsC+ swrA+ degQ+ amyE : : (P-rapP phrP cat) (yvzG/yvyD spc) McLoon et al. (2011)
GP736 trpC2 ΔsinR : : tet 8G5 sinR : : tet → 168
GP1517 trpC2 ΔepsA : : aphA3 this work
GP1518 trpC2 ΔepsB : : aphA3 this work
GP1519 trpC2 ΔepsAB : : aphA3 this work
GP1526 trpC2 epsA-3x FLAG spc pGP2127 → 168
GP1528 trpC2 sfp+ ermC epsC+ swrA+ degQ+ amyE : : (P-rapP phrP cat)(yvzG/yvyD Ωspc) ΔepsAB : : aphA3 GP1519 → AM373
GP1535 trpC2 sfp+ ermC epsC+ swrA+ degQ+ amyE : : (P-rapP phrP cat)(yvzG/yvyD Ωspc) ΔepsB : : aphA3 GP1518 → AM373
GP1540 trpC2 sfp+ ermC epsC+ swrA+ degQ+ amyE : : (P-rapP phrP cat)(yvzG/yvyD Ωspc) ΔepsA : : aphA3 GP1517 → AM373
GP1542 trpC2 xkdE : : Pxyl-epsB ermC pGP2129 → 168
GP1566 trpC2 ΔtkmA : : spc this work
GP1567 trpC2 ΔepsA : : aphA3 ΔtkmA : : spc GP1566 → GP1517
GP1568 trpC2 ΔepsA : : aphA3 ΔtkmA : : spc xkdE : : Pxyl-epsA ermC pGP2147 → GP1567
GP1575 ΔepsB : : aphA3 GP1535 → NCIB3610*
GP1577 ΔptkA ΔepsB : : aphA3 GP1535 → NRS2544*
GP1589 trpC2 sinR : : tet epsA-3x FLAG spc GP736 → GP1526
GP1600 ΔepsA : : aphA3 GP1540 → NCIB3610*
GP1602 tkmA : : spc GP1566 → NCIB3610*
GP1611 ΔepsA : : aphA3 tkmA : : spc GP1566 → GP1600*
GP1622 ΔsinR-tasA : : cat GP1672 → NCIB3610*
GP1623 ΔepsB : : aphA3 ΔsinR-tasA : : cat GP1672 → GP1575*
GP1624 ΔptkA ΔsinR-tasA : : cat GP1672 → NRS2544*
GP1625 ΔepsB : : aphA3 ΔptkA ΔsinR-tasA : : cat GP1672 → GP1577*
GP1626 ΔepsA : : aphA3 ΔsinR-tasA : : cat GP1672 → GP1600*
GP1627 ΔtkmA : : spc ΔsinR-tasA : : cat GP1672 → GP1602*
GP1628 ΔepsA : : aphA3 ΔtkmA : : spc ΔsinR-tasA : : cat GP1672 → GP1611*
GP1629 ΔepsA-O : : tet ΔsinR-tasA : : cat GP1672 → NRS2450*
GP1634 ΔepsB : : aphA3 ΔptkA xkdE : : Pxyl-epsB ermC GP1542 → GP1577*
GP1636 ΔepsA : : aphA3 ΔtkmA : : spc xkdE : : Pxyl-epsA ermC GP1568 → GP1611*
GP1637 ΔepsAB : : aphA3 GP1528 → NCIB3610*
GP1672 trpC2 ΔsinR-tasA : : cat this work
NRS2450 ΔepsA-O : : tet Ostrowski et al. (2011)
NRS2499 epsB (D81A+D83A) pNW329 → NCIB3610*
NRS2544 ΔptkA Kiley & Stanley-Wall (2010)

Arrows indicate construction by transformation.

Asterisks indicate construction by SPP1 phage transduction. BSGC represents the Bacillus genetic stock centre. Antibiotic resistance cassettes are: aphA3, kanamycin; cat, chloramphenicol; tet, tetracycline; spc, spectinomycin; ermC, erythromycin.