Figure 2.

Binding of MPK3 and MPK6 to Immobilized AZI1—Pull-Down Assay.

Lysates of E. coli expressing GST–MPK3 or GST–MPK6 (input) were incubated with chitin agarose without (‘mock’) or with AZI1 peptides immobilized by a chitin-binding-protein tag. Captured proteins were separated by SDS–PAGE and visualized by Coomassie Blue staining (top) or analyzed by immunoblotting with anti-GST antibody (bottom). The protein bands (‘f’) derive from AZI1 fusion proteins, which are partially cleaved into the intein–chitin-binding-protein tag (‘t’) and non-tagged AZI1 peptides (‘A’).