Figure 4.

ChIP-Seq Analysis of ASR5 Target Genes in Al-Treated Rice Plants.

(A) Western blot showing increased ASR5 protein levels in rice plants in response to Al. ASR5 was detected with anti-ASR5. (Cnt) indicates the control untreated plants, whereas (Al) indicates the plants that were treated with 450 μM AlCl₃ for 8h.

(B) Number and percentage of loci found in each binding region.

(C) Distribution of the binding sites. The x-axis displays the relative distance; the promoter region group is indicated in the top yellow bar; the coding region group is indicated by top blue and black bars; and the downstream region group is indicated by the top red bar. The y-axis displays the number of binding sites located in the different groups.