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Scatterplot analysis of 302 sera measured by enzyme-linked immunosorbent assay (ELISA)-immunoglobulin M (IgM) using rPap31 antigen. The vertical line separates immunofluorescent assay (IFA)-IgM-positive sera (sera nos. 1 to 34) from negative sera (sera nos. 35 to 302). The horizontal line indicates the cutoff value of the ELISA-IgM assay.
