Table 2.
Performance of the recombinant Pap31-based enzyme-linked immunosorbent assay (ELISA) compared with immunofluorescent assay (IFA) for detection of antibody against Bartonella bacilliformis
| No. ELISA-IgG | No. IFA positive (n) | No. IFA negative (n) | Total |
|---|---|---|---|
| Positive | 87 | 12 | 99 |
| Negative | 16 | 187 | 203 |
| Total | 103 | 199 | 302 |
| Performance of ELISA-IgG test at cutoff value* 0.915 (J = 0.79) | |||
| Sensitivity* 84.5% -Specificity* 94% | |||
| Positive likelihood ratio 14.1 -Negative likelihood ratio 0.16 | |||
| No. ELISA-IgM | No. IFA positive (n) | No. IFA negative (n) | Total |
| Positive | 30 | 40 | 70 |
| Negative | 4 | 228 | 232 |
| Total | 34 | 268 | 302 |
| Performance of ELISA-IgM test at cutoff value* 0.634 (J = 0.73) | |||
| -Sensitivity* 88.2% -Specificity* 85.1% | |||
| -Positive likelihood ratio 5.9 -Negative likelihood ratio 0.14 | |||
*
Using Youden index (J) = Max [sensitivity + specificity − 1] can range from 0 to 1.