Table 1.

Laboratory analytical methods used for biochemical tests, Kintampo, Ghana^*

Test	Analytical method used
ALT and AST	IFCC modified without pyridoxal phosphate
Amylase	2-Chloro-4-nitrophenyl-α-maltotrioside
Creatine kinase	IFCC, UV kinetic with imidazole buffer
GGT	L-γ-Glutamyl-3-carboxy-p-nitroanilide
LDH	UV kinetic, pyruvate to lactate
Total protein	Biuret
Albumin	Bromocresol green with succinate buffer
Total and direct bilirubin	Malloy-Evelyn modified, end-point
Cholesterol	Cholesterol oxidase/peroxidase
Glucose	Glucose oxidase/peroxidase
Iron	Chromazurol
Triglycerides	Lipase/glycerol kinase/glycerol peroxidase
Urea	Urease, UV kinetic
Creatinine	Jaffe kinetic
Uric acid	Uricase/peroxidase
Phosphorus	Phosphomolybdate
Electrolytes	Ion selective electrode, direct

^*ALT = alanine aminotransferase; AST = aspartate aminotransferase; IFCC = International Federation for Clinical Chemistry; GGT = γ-glutamyltransferase; LDH = lactate dehydrogenase; UV = ultraviolet.