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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Mol Microbiol. 2008 Apr 11;68(6):1428–1437. doi: 10.1111/j.1365-2958.2008.06245.x

Figure 3. The effect of the removal of laccase on cryptococcal prostaglandin production.

Figure 3

Panel A details the effect of the immunoprecipitation (IP) of laccase from cryptococcal lysates prior to the addition of AA. Inhibitory effect is displayed as a percent of control (the non-immunodepleted lysate + AA), which was set to 100 % (n = 4 ; * = p <0.001). Panel B demonstrates the effect of deleting the cryptococcal laccase genes on prostaglandin production into culture (when incubated with AA). Culture supernatants were taken from wild-type C. neoformans, lac1Δ, or lac2Δ strains that had been incubated for 2 hr. at 37°C in the presence of AA. Fungal prostaglandins were extracted from the culture supernatant into ethyl acetate and purified using RP-HPLC. Total prostaglandins were measured by EIA (n = 2; * = p <0.05).