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. 2014 Apr 2;9(4):e93690. doi: 10.1371/journal.pone.0093690

Figure 1. Effects of 18β-GA on cell proliferation and apoptosis.

Figure 1

A and B, MTS experiments showed that 18β-GA inhibited cell proliferation of A549 and NCI-H460 cells in a dose-dependent manner. Moreover, 18β-GA increased cytotoxicity of chemotherapeutic agent cisplatin. Data are expressed as mean±SD of three independent experiments done in triplicate. *p<0.05, **p<0.01. C, Western blot analysis demonstrated the increased cleaved PARP (85 kDa) with the decreased full length PARP (116 kDa) in A549 and NCI-H460 cells treated with 18β-GA. GAPDH (37 kDa) served as the loading control. D, Flow cytometric analysis of cell apoptosis. Percentage of cells in early or late apoptosis is provided in the lower right and upper right quadrants, respectively. Figures are the representative result selected from three independent experiments.