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. 2014 Apr 2;9(4):e93683. doi: 10.1371/journal.pone.0093683

Table 5. Bacterial strains and plasmids used in this study.

Strain or plasmid Description Source or reference
Strains
 Bacillus subtilis
 M168 Wild type C. Keel
 Escherichia coli
 DH5α, HB101 Laboratory strains [50]
 Pseudomonas protegens
 CHA0 Wild type [3]
 Cab57 Wild type This study
 Cab57retS ΔretS This study
Plasmids
pCR-Blunt II-TOPO Cloning vector, pUC ori; Kmr Invitrogen
pME497 Mobilizing plasmid, IncP-1, Tra, RepA(Ts); Apr [51]
pME3087 Suicide vector, ColE1 replicon, Mob; Tcr [52]
pME3087retS pME3087 containing a BamHI/HindIII 1.3-kb retS region containing a deletion of the retS gene; Tcr This study
pME6031 pACYC177-pVS1 shuttle vector; Tcr [49]
pME6060 Translational aprA’-‘lacZ fusion; Tcr [25]
pME6091 Transcriptional rsmZ-lacZ fusion; Tcr [7]