Table 5. Bacterial strains and plasmids used in this study.
Strain or plasmid | Description | Source or reference |
Strains | ||
Bacillus subtilis | ||
M168 | Wild type | C. Keel |
Escherichia coli | ||
DH5α, HB101 | Laboratory strains | [50] |
Pseudomonas protegens | ||
CHA0 | Wild type | [3] |
Cab57 | Wild type | This study |
Cab57retS | ΔretS | This study |
Plasmids | ||
pCR-Blunt II-TOPO | Cloning vector, pUC ori; Kmr | Invitrogen |
pME497 | Mobilizing plasmid, IncP-1, Tra, RepA(Ts); Apr | [51] |
pME3087 | Suicide vector, ColE1 replicon, Mob; Tcr | [52] |
pME3087retS | pME3087 containing a BamHI/HindIII 1.3-kb retS region containing a deletion of the retS gene; Tcr | This study |
pME6031 | pACYC177-pVS1 shuttle vector; Tcr | [49] |
pME6060 | Translational aprA’-‘lacZ fusion; Tcr | [25] |
pME6091 | Transcriptional rsmZ-lacZ fusion; Tcr | [7] |