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. 2014 Apr 2;9(4):e93446. doi: 10.1371/journal.pone.0093446

Table 4. Activation rates after mouse or rat oocytes collected 19(mouse) or mRECM (rat) medium supplemented with different concentrations of NaCl.

NaCl (mM) Mouse Rat
Oocytes observed % Oocytes activated Oocytes observed % Oocytes activated
0 131 45.2±4.3a 123 69.1±2.3a
25 130 44.4±3.7a - -
50 134 49.3±2.7a 130 21.1±1.8b
75 135 21.9±4.7b - -

a–b: Values with a common letter in their superscripts do not differ (P>0.05) in the same column. While rat oocytes were examined for spontaneous activation immediately after culture, mouse oocytes were treated with 5% ethanol for 10 min after culture and observed for activation 6 h later.