Table 4. Activation rates after mouse or rat oocytes collected 19(mouse) or mRECM (rat) medium supplemented with different concentrations of NaCl.
NaCl (mM) | Mouse | Rat | ||
Oocytes observed | % Oocytes activated | Oocytes observed | % Oocytes activated | |
0 | 131 | 45.2±4.3a | 123 | 69.1±2.3a |
25 | 130 | 44.4±3.7a | - | - |
50 | 134 | 49.3±2.7a | 130 | 21.1±1.8b |
75 | 135 | 21.9±4.7b | - | - |
a–b: Values with a common letter in their superscripts do not differ (P>0.05) in the same column. While rat oocytes were examined for spontaneous activation immediately after culture, mouse oocytes were treated with 5% ethanol for 10 min after culture and observed for activation 6 h later.