. 2014 Apr 2;9(4):e93446. doi: 10.1371/journal.pone.0093446

Table 5. Activation rates after mouse oocytes collected 13% ethanol for 10 min and cultured for 6 h in CZB medium supplemented with different concentrations of ouabain or NaCl.

Ouabain			NaCl
mM	Oocytes observed	% Oocytes activated	mM	Oocytes observed	% Oocytes activated
0	116	31.1±4.1^a	0	121	31.8±2.9^a
0.25	121	48.8±1.7^b	25	111	22.8±5.2^a
0.5	128	61±4.2^c	50	115	5.24±1.6^b
1	108	76.6±2.9^d	75	110	0.0±0.0^b
2	114	73.1±1.7^d	-	-	-

a–d: Values with a common letter in their superscripts do not differ (P>0.05) in the same column. Ouabain was used in place of bepridil because many mouse oocytes lysed when treated with bepridil following ethanol activation treatment.