Figure 1. Growth of LVS ΔfeoB′ in liquid and on agar.

Bacteria were inoculated in either iron rich TSB/c (A) or in che-CDM supplemented with FePPi at 2.5 μg/mL (High Fe) or 0.125 μg/mL (Low Fe)(B) and growth was followed as change in optical density at 600 nm (OD₆₀₀). Values were plotted as the means ± S.E. In Figure 1 C, bacterial strains were ten-fold serially diluted in che-CDM, spotted on MHA agar with (MHA+) and without (MHA-) iron supplementation and grown for 3 days at 37°C under aerobic conditions.