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. 2014 Apr 2;9(4):e93787. doi: 10.1371/journal.pone.0093787

Figure 2. Following release from growth arrest, mitotic germline nuclei re-enter S-phase before somatic endocycling cells.

Figure 2

A) IdU (S-phase marker) incorporation was gradually restored in the endocycling epithelial and mitotic germ line nuclei of GA animals that had been released into standard culture conditions. Scale bars = 50 μm. B) Pulse chases of CldU and IdU (see also materials and methods and Fig. S3) in GA animals (top panels), or in animals that had been released from GA for 2 (mid-panels) or 3 h (bottom panels), revealed that resumption of S-phase in the mitotic germline preceded that in somatic endocycling cells by 1 h. Scale bars = 50 μm. Insets depict zooms of the germline (Scale bars = 10 μm).