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| Primer | Primer sequence | Amplicon (bp) | |
| First-round PCR | TFPI2_2F | GGGGAGGTAGGTTTAATTTGG | 493 |
| TFPI2_Rd | TCCTAAACRCCCTATACAACTAAAA | ||
| Amplicon library preparation | TFPI2_LF | GTGTGGGGTATGAATTAGTTAGG | 212 |
| TFPI2_LR | AACCAACRAAATCCATAC |
