Table 1.
Summary of electrophysiological and Ca2+ imaging responses of urothelial cells to TRP agonists and pH changes
| Agonist | Electrophysiological Properties |
Ca2+ Imaging Properties |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| n | I@−80, pA/pF | I@+80, pA/pF | Erev, mV | Rectification ratio, −I@+80/I@−80 | n | ΔR/R, FAU | ||||||
| Background currents | 104 | −13.6±1.0 | −13.7±1.1 | 8.49±1.6 | 1.0±0.1 | |||||||
| 4α−PDD (1–5 μM) | 22 (22) | −32.2±6.6 | 42.4±7.0 | 8.9±2.8 | 1.4±0.1 | 48 (100) | 91.5±17.3 | |||||
| Capsaicin (1–100 μM) | 8 (31) | −31.1±15.2 | 31.5±12.2 | −5.5±6.8 | 0.8±0.1 | 82 (422) | 50.9±5.6 | |||||
| Icilin (50 and 100 μM) | 10 (14) | −54.9±16.2 | 53.4±16.9 | 8.7±1.4 | 0.9±0.1 | 13 (61) | 15.6±4.8 | |||||
| Menthol (5–200 μM) | 3 (7) | −15.3±9.5 | 14.4±8.1 | 10.9±2.1 | 1.0±0.3 | 30 (248) | 48.6±11.2 | |||||
| pH (AA) 5.5 | 10 (10) | −54.7±21.9 | 67.9±23.4 | −1.4±3.1 | 1.2±0.1 | NT | NT | |||||
| pH (HCl) 5.5 and 6.5 | 8 (14) | −16.9±8.1 | 15.2±6.9 | −5.2±0.9 | 0.9±0.1 | 37 (148) | 42.4±10.0 | |||||
Values are means ± SE; n, no. of agonist-responsive cells in patch-clamp and Ca2+ imaging experiments, respectively. Total number of cells tested is given in parentheses. TRP, transient receptor potential; I, current; FAU, fluorescence arbitrary units; NT, not tested; AA, acetic acid; Erev, reversal potential; 4α−PDD, 4α-phorbol-12,13 didecanoate.