Table 1. Sequences of RNA oligonucleotides.
Name | Sense Strand/Sense Primer (5'-3') | Antisense Strand/Antisense Primer (5'-3') |
Primers for Gene or 3'UTR Cloning | ||
YAP1 | TTCTCGAGGGAGATGGGATGAATATAGAAGG | GGTGTCTAGACCACAGGCAGCAGGAGAC |
YAP1-3'UTR | TTATCCCTCCTTTAAGTGAGATTCTCACAATTG | TTAAAGGAGGGATAAAGGAGTTATGGGT |
Primers for qRT-PCR | ||
YAP1 primers | GAACTCAGCATCAGCCATGA | CTACGGAGAGCCAATTCCTG |
Cyclin E primers | CACCCTCTCCTGCAACCTAA | TGGTGCAACTTTGGTGGATA |
DIAP1 primers | GCCATAACAACTGCTGCTGA | TCTCTTTCAAGGCAGGCAAT |
GAPDH primer | AGGCTGAGAACGGGAAACTTG | CACCTGCATCTGCCCATTTG |