Figure 9. Foxo4 overexpression abolished anti-oxidative effects of α-MSH in retinal vascular endothelial cells.

In the CM-H2DCFDA assay, the fluorescence intensities of cell groups was measured and cell images were captured under a fluorescence microscope. Representative images are shown in (A). The fluorescence intensity was normalized to the total protein concentration of each sample (B). n = 5–10/group, * p<0.05, ** p<0.01, *** p<0.001.