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. 2014 Apr 2;9(4):e93257. doi: 10.1371/journal.pone.0093257

Figure 4. ATP6V0C regulates basal and stress-induced metabolism of alpha synuclein.

Figure 4

Representative western blot for α-syn (A) from lysates following nucleofection and subsequent treatment for 48 h with 0–100 nM bafilomycin A1 (BafA1), indicating α-syn high molecular weight (HMW) species (>50 kDa, suggesting multimeric species) and α-syn monomer (∼17 kDa). Blots were stripped and re-probed for actin (42 kDa) to normalize for gel loading. Data from at least six independent experiments are presented graphically in panels B–E. Within groups comparisons of BafA1 concentration responsiveness (Non-target siRNA, open columns, left; ATP6V0C siRNA, filled columns, right) were determined for α-syn HMW species (C) or monomer (D) by expressing mean ± SEM band intensities relative to actin loading control (results of one-way ANOVA were not significant, p>0.05). Comparisons between groups (Non-target vs. ATP6V0C siRNA) for α-syn HMW species (D) or monomer (E) were determined for each concentration of BafA1 by expressing mean ± SEM fold changes for each ATP6V0C siRNA condition relative to its companion Non-target control. #p<0.05 vs. corresponding Non-target siRNA control using one-sample t-test.