Figure 3.

Specific isolation and characterization of human subventricular zone neural progenitor cells using magnetic beads. (A): In the CD271⁻ fraction, pan-GFAP-positive cells (red) are present, but not GFAPδ-positive cells (green). In the CD271⁺ fraction, all GFAP-pan (A)- and nestin (B)-positive cells are also positive for GFAPδ. GFAPδ⁺/CD271⁺ cells are positive for Sox2 (green) and nestin (red) (C) and GFAPδ (green) (D), and some cells express GFAPα (red), vimentin (red), and pan-GFAP (green) (E). (F): GFAPδ⁺/CD271⁺ cells express glutamine synthetase (red) but no S100b (green), and (G) some cells are positive for pHH3 (green), whereas βIII-tubulin (red) is not expressed. Scale bars represent 100 μm. Abbreviations: GFAP, glial fibrillary acidic protein; Sox2, sex-determining region Y-box 2.