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. 2014 Mar 6;3(4):470–480. doi: 10.5966/sctm.2013-0038

Figure 6.

Figure 6.

In adherent human fetal neural progenitor cells (NPCs), CD271 is not specifically expressed on GFAPδ-positive NPCs. (A): Many adherent NPCs derived from human fetal brain, 14 weeks of gestation, are GFAPδ positive. Some of these cells are also positive for CD271 (arrows), whereas other cells expressing CD271 do not express GFAPδ (arrow) (B). (C): Both CD271− and CD271+ human fetal NPCs are able to form neurospheres in vitro. (D): Double staining of GFAP-pan (green) plus βIII-tubulin (red) in CD271− and CD271+ human fetal NPCs that were differentiated for 7 days. Neurospheres were dissociated and plated onto laminin-coated wells without growth factors and in the presence of 2% fetal calf serum and 100 ng/ml brain derived neurotrophic factor (BDNF). Scale bars = 100 μm. Abbreviation: GFAP, glial fibrillary acidic protein.