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. 2014 Mar 3;3(4):416–423. doi: 10.5966/sctm.2013-0105

Figure 2.

Figure 2.

In vitro and in vivo differentiation capacity of human embryonic stem cell (hESC), NHDF-derived control-induced pluripotent stem cell (iPSC), and HLHS-iPSC lines. (A): In vitro differentiation capacity to all three germ layers demonstrated by immunocytochemical staining with an anti-AFP antibody (endoderm), anti-CD31 antibody (mesoderm), and anti-βIII-tubulin antibody TUJ1 (ectoderm). DAPI staining of nuclei is shown in blue. Shown is a representative example of at least three independent experiments. Scale bar = 100 μm. (B): In vivo differentiation of hESC line H9 (upper panel), NHDF1-iPSCs (middle panel), and HLHS1-iPSCs (lower panel). All three pluripotent stem cell lines produced teratomas containing structures representative of each germ layer, notably neuroepithelium (Ba, Bd, Bg) (ectoderm), kidney (Bb, Be)/gut (Bh) wall (both endoderm), and cartilage (Bc, Bf, Bi) (mesoderm). Histological staining: Masson’s trichrome for iPSC-HLHS mesoderm and Weigert’s stain for all other sections. Scale bars = 150 µm (NHDF1-iPSCs and iPSC-HLHS) and 75 µm (all others). Abbreviations: AFP, α-fetoprotein; DAPI, 4′,6-diamidino-2-phenylindole; HLHS, hypoplastic left heart syndrome; NHDF, neonatal human dermal fibroblast.