. Author manuscript; available in PMC: 2014 Apr 2.

Published in final edited form as: J Proteome Res. 2007 Oct 4;6(11):4374–4387. doi: 10.1021/pr070446f

Table 2.

Heparan Sulfate Content and Disaccharide Analysis

cell type	total HS (ng/10⁶cell)	HS (unsaturated disaccharide, %)^a
cell type	total HS (ng/10⁶cell)	NAc^b	NS	NAc6S	2SNAc	NS6S	2SNS	2SNAc6S	2SNS6S
mESC	12.1	60.5	18.2	4.5	0.8	2.6	10.2	0.0	3.2
EB	57.5	59.1	17.5	6.1	0.4	4.2	10.6	0.0	2.1
ExE	93.9	49.3	22.4	6.9	0.8	4.8	8.9	0.0	6.9

The degree of sulfation calculated from the disaccharide analysis was 0.66 sulfo groups/disaccharide for mESE, 0.60 sulfo groups/disaccharide for EB, and 0.78 sulfo groups/disaccharide for ExE.

Abbreviations for HS disaccharide used in Figure 1 and throughout the text.