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. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: Nature. 2014 Jan 30;505(7485):706–709. doi: 10.1038/nature12946

Extended Data Figure 8. Footprinting validation of a RiboSNitch in WSB1 transcript identified by PARS.

Extended Data Figure 8

a, The sequence of a portion of the WSB1 transcript containing the RiboSNitch was shown. The RiboSNitch is in red. Gel analysis of 2 fragments of WSB1 RNA T and C alleles using RNase V1 (lanes 5 (Mother), 6 (Father)), S1 nuclease (lanes 7 (Mother), 8 (Father)), and SHAPE probing ((lanes 9 (Mother), 10 (Father)). Additionally, sequencing lanes (lanes 1,2), DMSO uncut lanes (lane 3 (Mother), lane 4 (Father)) are also shown. Black arrow indicates the change in structure between the Father and Mother alleles. b, Fraction of S1 sequencing reads over total S1 sequencing reads in the region, across the RiboSNitch for both Father and Mother. c,d, SAFA quantification of the RNA footprinting of both alleles using S1 nuclease (c) and SHAPE (d).