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. 2014 Mar 19;15(1):133–140. doi: 10.4142/jvs.2014.15.1.133

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© 2014 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — The number of TRAP-positive multinucleated osteoclasts formed with or without M-CSF, RANKL, and 1α,25-(OH)₂D₃. No osteoclasts were formed in group A (RAW264.7 cells cultured without any cytokines). M-CSF and RANKL induced the formation of TRAP-positive multinucleated osteoclasts (groups B~E). In addition, 1α,25-(OH)₂D₃ increased the number of TRAP-positive multinucleated osteoclasts (groups C ~E). ^**p < 0.01 vs. group A, ^†p < 0.05 vs. group B, and ^††p < 0.01 vs. group B.