Skip to main content
. 2014 Mar 19;15(1):27–33. doi: 10.4142/jvs.2014.15.1.27

Fig. 2.

Fig. 2

Immunohistochemistry specific for DCX in the dentate gyrus. DCX-positive neuroblasts were detected in the subgranular zone of the dentate gyrus. The number of DCX-immunoreactive neuroblasts was decreased in the LFD-RSG group compared to the LFD-Vehicle group. DCX-positive neuroblasts were rarely seen in the HFD-Vehicle group unlike the LFD-Vehicle group. The number of DCX-immunoreactive neuroblasts in the dentate gyrus was increased in the HFD-RSG group compared to the HFD-Vehicle group. (A and B) LFD-Vehicle. (C and D) LFD-RSG. (E and F) HFD-Vehicle. (G and H) HFD-RSG groups. (I) Relative number of DCX-immunoreactive cells in the LFD-Vehicle, LFD-RSG, HFD-Vehicle, and HFD-RSG groups (n = 7 per group; *p < 0.05, Vehicle vs. RSG groups; †p < 0.05, LFD vs. HFD groups). All data are expressed as the mean ± SEM. (J) Western blot analysis of DCX levels in the dentate gyrus of the LFD-Vehicle, LFD-RSG, HFD-Vehicle, and HFD-RSG groups. Relative optical density (ROD) of the bands is expressed as percentages (n = 5 per group; *p < 0.05, Vehicle vs. RSG groups; †p < 0.05, LFD vs. HFD groups). Data are presented as the mean ± SEM. Scale bars = 25 µm (B, D, F, and H) or 50 µm (A, C, E, and G).