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. 2004 Feb 12;101(15):5339–5346. doi: 10.1073/pnas.0308709101

Fig. 1.

Fig. 1.

Generation of mPer2Luc knockin mice. (A) Diagram of the mPer2 locus, targeting vector, and targeted knockin allele. Exons are indicated by filled blocks with numbers. E, EcoRI; DT, diphtheria toxin A chain; Neo, neomycin resistance gene; triangle, loxP site. (B) Southern blot of DNA from F2 animals after digestion with EcoRI. The 600-bp 3′ external probe (A) detects a 15.9-kb WT fragment and a 10.2-kb targeted fragment. + indicates WT; L indicates luc knockin allele. (C) PCR genotyping of F2 animals. Agarose gel electrophoresis reveals the presence of a 230-bp WT (+) allele and a 680-bp knockin allele (L). (D) Northern blot of total RNA extracted from mouse brain probed with a 1.4-kb mPer2 partial cDNA fragment. In contrast to the 7.5-kb WT (+) allele, the larger 11.1-kb band represents the transcript from the targeted (L) allele. (E) Western blot of WT (+/+), mPer2Luc heterozygote (+/L), and homozygote (L/L) mouse.