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Microscopic examination. (A) Solid area composed of a sheet of polygonal or cuboidal clear cells with intermediate nuclear grade. (H&E × 200) (B) Tubular structure lined by clear columnar cells having subnuclear or supranuclear vacuoles, characterized by fetal gut appearance. (C) Diffuse positive reaction for AFP in the cytoplasm. (D) Diffuse positive reaction for GPC3 in the cytoplasm. (E) Focal positive reaction for SALL4 in the nucleus.
