Table 1.
Primers used for real-time RT-PCR
| Primer | Sequences |
|---|---|
| hNrf2 |
Forward primer,5′- CATCCAGTCAGAAACCAGTGG |
| Reverse primer ,5′- GCAGTCATCAAAGTACAAAGCAT | |
| hKeap1 |
Forward primer, 5′-CCTTCAGCTACACCCTGGAG |
| Reverse primer, 5′-CATGACCTTGGGGTGGATAC | |
| hGR |
Forward primer, 5′-CACGGA GGAGCTGGAGAAC |
| Reverse primer, 5′- CGACAAAGTCTTTTTAACCTCCTT | |
| hTR |
Forward primer, 5′-CAGACG GGGAGGCTTTTC |
| Reverse primer, 5′- CCGAGAGCGTTCCTTTCA | |
| hHO-1 |
Forward primer, 5′- TCCTGGCTCAGCCTCAAATG; |
| Reverse primer, 5′- CGTTAAACACCTCCCTCCCC | |
| hr-GCSc |
Forward primer, 5′-CTGTTGCAGGAAGGCATTGAT |
| Reverse primer, 5′- TTCAAACAGTGTCAGTGGGTCTCT | |
| hr-GCSm |
Forward primer, 5′- GGCACAGGTAAAACCAAATAGTAAC |
| Reverse primer, 5′- CAAATTGTTTAGCAAATGCAGTCA | |
| hNQO1 |
Forward primer, 5′- GGGATCCACGGGGACATGAATG |
| Reverse primer, 5′- ATTTGAATTCGGGCGTCTGCTG | |
| hUGT1A1 |
Forward primer, 5′-TAAGTGGCTACCCCAAAACG |
| Reverse primer, 5′- TCCAGCTCCCTTAGTCTCCA | |
| hUGT1A10 |
Forward primer, 5′- CGTGTTCTGGGTGGAGTTTG |
| Reverse primer, 5′- TTTTCCCCAAGCATTTCCGG | |
| hGAPDH |
Forward primer, 5′- TGCACCACCAACTGCTTAGC |
| Reverse primer, 5′- GGCATGGACTGTGGTCATGAG | |
| mGR |
Forward primer, 5′- AGTGCACTCGGAATTCATGC |
| Reverse primer, 5′- CAATCAGGATGTGTGGAGCG | |
| mTR |
Forward primer, 5′- ATCCACAAACAGCGAGGAGA |
| Reverse primer, 5′- TTGGTCTGCTCTTCATCCGT | |
| mHO-1 |
Forward primer, 5′- GCCACCAAGGAGGTACACAT |
| Reverse primer, 5′- GCTTGTTGCGCTCTATCTCC | |
| mr-GCSc |
Forward primer, 5′- GGCCACTATCTGCCCAATTG |
| Reverse primer, 5′- TGTTCTTCAGAGGCTCCAGG | |
| mr-GCSm |
Forward primer, 5′- GGAGGGGCTCTTAACTCCAG |
| Reverse primer, 5′- CTCAACACAGTGCCGAACAA | |
| mNQO1 |
Forward primer, 5′- ACAGGTGAGCTGAAGGACTC |
| Reverse primer, 5′- GTTGTCGTACATGGCAGCAT | |
| mUGT1A1 |
Forward primer, 5′- GGAGGCTGTTAGTGTTCCCT |
| Reverse primer, 5′- CCGTCCAAGTTCCACCAAAG | |
| mUGT1A10 |
Forward primer, 5′- GACTCGGGCATTCATCACAC |
| Reverse primer, 5′- GCGCATGATGTTCTCCTTGT | |
| mTNF-a |
Forward primer, 5′- GACCCCTTTACTCTGACCCC |
| Reverse primer, 5′- AGGCTCCAGTGAATTCGGAA | |
| mIL-1b |
Forward primer, 5′- ACTCATTGTGGCTGTGGAGA |
| Reverse primer, 5′- TTGTTCATCTCGGAGCCTGT | |
| mIL-6 |
Forward primer, 5′- CTGCAAGAGACTTCCATCCAGTT |
| Reverse primer, 5′- GAAGTAGGGAAGGCCGTGG | |
| mGAPDH |
Forward primer, 5′- CTCCCACTCTTCCACCTTCG |
| Reverse primer, 5′- CCACCACCCTGTTGCTGTAG |