Table 1.

Validation of auxiliary subunits and accessory proteins.

Proteins	Kv4.2-IP PAF	Kv4 CoIP	Δ Ipeak	Δ Functional properties
Auxiliary subunits
KChIP1	–	Brain	↑ 7.5-fold	Slowed inactivation; rightward shift in SSI; accelerated recovery; accelerated closing (An et al., 2000; Holmqvist et al., 2002; Morohashi et al., 2002)
KChIP2	1.7	Brain	↑ 5.5-fold
KChIP3	1.4	Brain	↑ 5.8-fold
KChIP4	5.6	Brain	↑ 2- to 3-fold
DPP6	5.3	Brain	↑ 4- to 18-fold	Accelerated activation and inactivation; leftward shifts in SSI and G-V relationship; accelerated recovery; accelerated channel closing (Nadal et al., 2003,2006; Jerng et al., 2004b; Zagha et al., 2005)
DPP10	2.0	Brain	↑ 5.9-fold
Accessory proteins
Kvβ1	0.7	Brain	↑ 0.2- to 3-fold	Moderate acceleration of inactivation; moderate to no effect on SSI; no effect on recovery; Kv1 preference (Nakahira et al., 1996; Yang et al., 2001; Wang et al., 2003; Aimond et al., 2005)
Navβ1	0.8	Brain Heart	↑ 0.3- to 2-fold	Inconsistent effects reported (Chabala et al., 1993; Deschenes and Tomaselli, 2002; Deschenes et al., 2008; Hu et al., 2012; Marionneau et al., 2012a)
MiRP1	–	In vitro	Variable	Inconsistent effects reported (Zhang et al., 2001; Deschenes and Tomaselli, 2002; Radicke et al., 2006)
KChAP	–	Heart	↑ 0.8-fold	None (Kuryshev et al., 2000,2001)
NCS-1	–	Heart	↑ 0.5-fold	Inconsistent effects reported (Nakamura et al., 2001; Guo et al., 2002; Ren et al., 2003)
PSD-95	–	In vitro	↑ 2-fold	None (Wong et al., 2002; Wong and Schlichter, 2004)
Filamin	–	Brain	↑ 2.5-fold	None (Petrecca et al., 2000)
Kinesin isoform Kif17	–	Brain	ND	ND (Chu et al., 2006)

PAF, protein abundance factor; PAF (Kv4.2) = 2.1 (Marionneau et al., 2011); (–), not detected; ND, not determined; SSI, steady-state inactivation; G-V, conductance-voltage.

Other proteins (Gabra-6, Gpr158, Prkcb1) have not undergone validation.